Whole-Genome Sequencing of Sinocyclocheilus maitianheensis Reveals Phylogenetic Evolution and Immunological Variances in Various Sinocyclocheilus Fishes

Whole-Genome Sequencing of Sinocyclocheilus maitianheensis Reveals Phylogenetic Evolution and Immunological Variances in Various Sinocyclocheilus Fishes

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An adult Sinocyclocheilus maitianheensis, a surface-dwelling golden-line barbel fish, was Left Link Cover collected from Maitian river (Kunming City, Yunnan Province, China) for whole-genome sequencing, assembly, and annotation.We obtained a genome assembly of 1.7 Gb with a scaffold N50 of 1.4 Mb and a contig N50 of 24.

7 kb.A total of 39,977 protein-coding genes were annotated.Based on a comparative phylogenetic analysis of five Sinocyclocheilus species and other five representative vertebrates with published genome sequences, we found that S.maitianheensis is close to Sinocyclocheilus anophthalmus (a cave-restricted species with similar locality).

Moreover, the assembled genomes of S.maitianheensis and other four Sinocyclocheilus counterparts were used for a fourfold degenerative third-codon transversion (4dTv) analysis.The recent whole-genome duplication (WGD) event was therefore estimated to occur about 18.1 million years ago.

Our results also revealed a decreased tendency of copy number in many important genes related to immunity and apoptosis in cave-restricted Sinocyclocheilus species.In summary, we report the first genome assembly of S.maitianheensis, which provides Deodorant a valuable genetic resource for comparative studies on cavefish biology, species protection, and practical aquaculture of this potentially economical fish.